Let's Talk Micro

Luis Plaza

<p>Clinical Microbiology explained in simple terms, suitable for students and microbiologists alike. Hosted by Luis Plaza, a Medical Laboratory Scientist, he also talks about his experiences as a clinical microbiologist.</p>

  • 1 minute 9 seconds
    Quick Update from Let's Talk Micro

    Luis here with a quick update! We're taking a short break and will return the week of March 23rd. During this time, it's a great opportunity to explore the archive—Let's Talk Micro has over 200 episodes covering many topics in clinical microbiology. New episodes will be back soon. Thanks for listening and for being part of the Let's Talk Micro community.

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    10 March 2026, 11:00 pm
  • 57 minutes 27 seconds
    225: Too Many Bugs, One Culture: A CMPH Guide to Wound Cultures

    Wound cultures are some of the most complex specimens encountered in the clinical microbiology laboratory. Unlike more straightforward cultures, wound specimens often contain multiple organisms, making it challenging to determine which microbes are clinically significant and which represent colonization.

    In this episode of Let's Talk Micro, Luis is joined by Dr. Christopher Doern and Dr. Alexandra Bryson to discuss the wound culture guidelines from the Clinical Microbiology Procedures Handbook (CMPH) and how they can help laboratorians navigate these challenging cultures.

    Together, they explore practical approaches to interpreting polymicrobial wound cultures, deciding which organisms to work up, and balancing thorough reporting with antimicrobial stewardship. The discussion also covers specimen collection challenges, the role of Gram stains in evaluating specimen quality, guidance on working up common wound pathogens, and strategies to streamline laboratory workflows.

    Whether you're a student, a new microbiology technologist, or an experienced laboratorian, this episode offers practical insights to help you confidently approach wound cultures and apply CMPH guidance at the bench.

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    6 March 2026, 12:00 am
  • 13 minutes 57 seconds
    224: Micro Minutes: Sometimes Gram Stains Lie

    The Gram stain is one of the most trusted tools in clinical microbiology—but it doesn't always tell the full story.

    In this Micro Minutes episode, we break down why Gram stains can sometimes be misleading and how factors like culture age, antibiotic exposure, decolorization, morphology, and arrangement can change what you see under the microscope. We also touch on classic troublemakers like Acinetobacter and tiny coccobacilli such as Pasteurella and Haemophilus, and how careful observation can keep you from jumping to the wrong conclusion.

    Short, focused, and straight from the bench—this episode is all about learning how to interpret Gram stains beyond just purple and pink.

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    27 February 2026, 12:00 am
  • 51 minutes 42 seconds
    223: SuperStat to Storytime: Building the Future of Laboratory Medicine

    In this episode of Let's Talk Micro, we step beyond the microbiology bench to talk laboratory medicine, advocacy, and storytelling with fellow medical laboratory scientists and podcasters Stephanie Whitehead and Aaron Odegard.

    We explore how their work on the SuperStat Podcast is helping amplify the voice of the laboratory through conversations on leadership, access, and visibility. The discussion also highlights Little Lab Learners: ABCs of Laboratory Medicine, a children's book created to introduce laboratory medicine early and inspire future lab professionals.

    Together, we talk about lab career paths, the impact of automation and the pandemic on lab visibility, and why advocacy doesn't look the same for everyone—but matters now more than ever. This episode is a reminder that the lab's story doesn't end at the bench—it's one we all help tell.

    📘 Learn More About Little Lab Learners

    Little Lab Learners: ABCs of Laboratory Medicine is a children's book created to introduce laboratory medicine early and help kids see lab professionals as scientists, problem-solvers, and essential members of healthcare.

    Learn more about the book and the Little Lab Learners series here: 👉 https://labcares.org/Little_Lab_Learners/

    🎙️ Check Out the SuperStat Podcast

    Listen to the SuperStat Podcast on Spotify: 👉 https://open.spotify.com/show/5wYrCFh375KclnCFY8YyN2?si=b681fc3748df48e4

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    20 February 2026, 12:00 am
  • 48 minutes 45 seconds
    222: Parasites, Pediatrics, and the Lab: A Study from Guinea, West Africa

    In this episode of Let's Talk Micro, Luis is joined by Dr. Joel Mortensen to discuss a study examining intestinal parasites in pediatric patients in Guinea, West Africa.

    They dive into the real-world challenges of diagnosing parasitic infections in resource-limited settings, including reliance on stool microscopy, the use of rapid antigen tests, and how seasonal changes influence infection rates. Dr. Mortensen walks us through the study design, key findings, and what the data revealed about protozoal infections, as well as the role of microscopy in identifying helminths in this setting.

    This conversation highlights the critical role of clinical microbiology in global health, the importance of diagnostic context, and how laboratory data can directly inform patient care and public health strategies in underserved regions.

    Check out Dr. Joel Mortensen's previous episodes

    If you enjoyed this conversation, be sure to check out previous episodes featuring Dr. Mortensen, where we dive deeper into his work in Guinea, laboratory capacity building, and global health challenges in resource-limited settings:

    These episodes provide important background on the hospital, training efforts, and the broader impact of this work.

    🔗 Other Links

    Learn more about Sacre Coeur Hospital and the pediatric care and laboratory work being done in Guinea: 👉 https://www.hopeignited.org/sacre-coeur

    📩 Want to get involved or learn more?

    If you're interested in learning more about this work, collaborating, or supporting efforts in Guinea, you can reach out directly to Dr. Joel Mortensen:

    📧 [email protected]

    You can also contact me at [email protected], and I'm happy to help connect you.

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    13 February 2026, 12:00 am
  • 35 minutes 46 seconds
    221: Beyond the Plate: Your Isolates and Genomic Epidemiology

    Genomic epidemiology is reshaping how we detect, investigate, and control infectious disease outbreaks—but what does that actually look like from the bench to public health? In this episode of Let's Talk Micro, Luis is joined by genomic epidemiologist Krisandra Allen for a practical, case-based conversation on how pathogen sequencing data is generated, analyzed, and paired with epidemiologic information to answer questions traditional methods can't. They discuss foodborne and hepatitis A outbreaks, geographically dispersed clusters, and how sequencing is being integrated into routine public health workflows, while highlighting the essential role of clinical microbiology labs in specimen submission and data quality. A timely episode for microbiologists, public health professionals, and trainees curious about how their lab work feeds into real-world surveillance and outbreak response.

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    6 February 2026, 12:00 am
  • 1 hour 8 minutes
    220: New Clinical Microbiology Certification Pathway at UF

    In this episode of Let's Talk Micro, Luis is joined by faculty and collaborators from the University of Florida to discuss their new Clinical Laboratory Microbiologist (CLM) program — the first NAACLS-approved, microbiology-only certification pathway in the United States.

    They share how the program was created to address the growing shortage of clinical microbiologists and provide a direct pathway for microbiology graduates and working professionals to enter the clinical laboratory.

    The conversation covers:

    • Gaps in the current workforce and training pipeline

    • The hybrid model combining online coursework, hands-on bootcamp labs, and local clinical internships

    • Real-world training with clinical lab technology such as MALDI-TOF, PCR, and blood culture systems

    • Preparation for the ASCP categorical microbiology certification exam

    • The strong nationwide interest in the program

    The episode closes with a fun discussion on everyone's favorite microbes.

    Whether you're a student, lab professional, or educator, this episode offers insight into the future of clinical microbiology training.

    Additional resources:

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    30 January 2026, 12:00 am
  • 59 minutes 29 seconds
    219: Tick-Borne Diseases: The Lab and Diagnostics

    Tick-borne diseases continue to expand across North America, but diagnosing them in the clinical laboratory remains complex—especially when timing between symptom onset and testing isn't considered.

    In this episode of Let's Talk Micro, Luis is joined by Kyle Rodino, Assistant Professor of Pathology and Laboratory Medicine at the University of Pennsylvania and Assistant Director of Microbiology, for a lab-focused discussion on tick-borne disease diagnostics.

    They cover major bacterial, parasitic, and viral causes of tick-borne disease, and walk through how molecular testing, serology, and microscopy are used at different stages of illness. The conversation highlights why test selection and timing matter, common diagnostic challenges—particularly with Babesia and Lyme disease testing—and how laboratories can support better diagnostic decision-making.

    A practical episode for microbiologists, laboratorians, and clinicians navigating real-world testing challenges in the lab.

    Additional resources: Update on North American tick-borne diseases and how to diagnose them https://doi.org/10.1128/jcm.00807-23

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    23 January 2026, 12:00 am
  • 1 hour 9 minutes
    218: Metagenomics in Clinical Microbiology

    In this episode of Let's Talk Micro, we break down metagenomic next-generation sequencing (mNGS) and how it's changing the way we diagnose complex infectious diseases.

    I'm joined by Steve Miller, MD, PhD—Chief Medical Officer at Delve Bio—to discuss how unbiased metagenomics moved from research labs into real-world clinical practice.

    We cover what metagenomics is, how it differs from targeted PCR and sequencing, and where it adds the most value—especially in meningitis and encephalitis, immunocompromised patients, and cases where routine testing comes back negative.

    Dr. Miller shares insights from years of clinical experience, including how mNGS can improve diagnostic yield, shorten time to diagnosis, guide targeted therapy, and reduce unnecessary testing and hospital stays. We also touch on challenges like cost, result interpretation, diagnostic stewardship, and where metagenomics is headed next—including its role in public health and emerging infections.

    Links & Resources

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    16 January 2026, 12:00 am
  • 12 minutes 24 seconds
    217: Micro Minutes: Don't Let PEA Fool You

    PEA agar is often treated as "the gram-positive plate," but that shortcut can lead to real bench-level mistakes.

    In this MicroMinutes episode, we break down what phenylethyl alcohol agar actually does, why growth on PEA does not automatically mean an organism is gram-positive, and how selective media can change colony appearance and behavior.

    You'll hear about:

    • What PEA inhibits — and what it doesn't

    • Why Pseudomonas aeruginosa can grow quite well on PEA

    • How colony morphology and hemolysis may differ from blood agar

    • Why Lancefield grouping should never be performed directly from PEA

    PEA is a powerful recovery tool — but only when you understand its limitations. Short, focused, and straight from the bench, this is your Micro minutes.

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    19 December 2025, 12:00 am
  • 1 hour 7 minutes
    216: AMR Summit 2025: One Health, One Fight

    In this special episode, I'm joined by three leaders who helped shape the conversation at the 2025 Antimicrobial Resistance (AMR) Summit in Detroit: Dr. Lauren Hunt, Dr. Linoj Samuel, and Dr. Robert Tibbetts. Together, we explore how this intimate, multidisciplinary summit brought together clinical microbiology, infectious diseases, stewardship, public health, policy, and veterinary medicine to tackle the growing challenges of antimicrobial resistance.

    We break down the key themes that emerged throughout the meeting—shared accountability, One Health collaboration, diagnostic innovation, payer perspectives, and the urgent need for equitable access to testing. You'll hear about surprising insights, personal stories that brought AMR's impact into sharp focus, and the collaborative projects already taking root as a result of this year's summit.

    Whether you're on the bench, managing stewardship programs, shaping policy, or simply passionate about the future of diagnostics, this episode offers a behind-the-scenes look at the conversations and partnerships driving AMR action forward.

    Thank you to Lauren, Linoj, and Robert for joining me—and to everyone committed to advancing the fight against AMR. One Health, one fight.

    Additional Resources from the 2024 AMR Summit

    If you'd like to explore more background from last year's summit, here are two excellent resources:

    Want to Contribute or Get Involved?

    If you're interested in contributing ideas, collaborating on AMR initiatives, or learning more about future AMR Summits, you can reach out directly to:

    Dr. Lauren Hunt

    📧 [email protected]

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    12 December 2025, 12:00 am
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